A Loop-Mediated Isothermal Amplification (Lamp)-Based Point-of-Care System for Rapid on-Site Clinical Detection of Sars-Cov-2 Viruses

In the ongoing COVID-19 pandemic, sensitive and rapid on-site detection of the SARS-CoV-2 coronavirus has been one of crucial objectives. A point-of-care (PoC) device called PATHPOD for quick, on-site detection of SARS-CoV-2 employing a real-time reverse-transcription loop-mediated isothermal amplification (RT-rLAMP) reaction on a polymer cartridge. The PATHPOD consists of a standalone device (weighing under 1.2 kg) and a cartridge, and can identify 10 distinct samples and 2 controls in less than 50 minutes. The PATHPOD PoC system is fabricated and clinically validated for the first time in this work © 2023 IEEE.

MICROFLUIDIC DIAGNOSTIC AND POINT OF CARE DETECTION SYSTEM FOR SARS-COV-2 FROM SALIVA

We present a nucleic acid-based point-of-care diagnostic for the detection of the SARS-CoV-2 from saliva using an additively manufactured microfluidic cartridge. The assay uses reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) to detect the presence of SARS-CoV-2 RNA on-cartridge in a point-of-care optical detection system based on a smartphone. We show positive results within the 10-30 minutes range and integrated biological controls on the cartridge. We demonstrate the microfluidic diagnostic with human patient samples, with results that are consistent with the off-cartridge validation. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Undergraduate student’s misconception about projectile motion after learning physics during the Covid-19 pandemic era

This research aimed to describe undergraduate students' misconception about projectile motion after learning physics during the Covid-19 pandemic era. This research was qualitative research with a descriptive method. The subjects were 52 first-year undergraduate students who took physics courses. Data collecting methods used in this research were a test, questionnaires, and interviews. The test was taken from Physics by Giancoli with an additional question about certainty of response index (CRI). Data from the test were analyzed by categorizing it into lack of knowledge, knowledge of correct concepts, and misconception while open-ended questionnaires and interviews were used to help to clarify the condition. The test results indicated that 5.13% of students in lack knowledge, 28.85% the knowledge of correct concepts, and 66.02% in misconception. The questionnaire responses showed that students learned physics via online meeting with direct instruction model and ask-answer method, exercised with only applied problem (C3), and virtual practicum. The interviews showed that only a few of the students learned physics and responded to the lecturer during the online meeting. The results are that the majority of first-year undergraduate students are in misconception after learning physics during the Covid-19 pandemic era and need remedial learning about projectile motion. © 2021 Institute of Physics Publishing. All rights reserved.